
- 品名: 普諾賽SU-DHL-4 (人B淋巴瘤細(xì)胞)
- 型號(hào): CL-0692
- 產(chǎn)品詳情
- 規(guī)格參數(shù)
產(chǎn)品信息
名稱 | SU-DHL-4 (人B淋巴瘤細(xì)胞) (STR鑒定正確) |
別稱 | SUDHL4; Sudhl4; SUDHL-4; Sudhl-4; SuDHL 4; SUD-4; SUD4; SU4; Stanford University-Diffuse Histiocytic Lymphoma-4; DHL-4; DHL4 |
背景描述 | Established from the peritoneal effusion of a 38-year-old man with B-NHL (diffuse large cell, cleaved cell type; originally described as "diffuse histiocytic lymphoma") in 1975; cell line carries EZH2 Y641S mutation; assigned to GCB-like lymphoma subtype (germinal center B-cell). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
種屬 | 人類 |
年齡(性別) | 男性,38歲 |
組織來(lái)源 | 腹腔積液 |
生長(zhǎng)特性 | 懸浮細(xì)胞 |
細(xì)胞形態(tài) | 淋巴母細(xì)胞樣 |
細(xì)胞類型 | 腫瘤細(xì)胞 |
腫瘤類型 | 淋巴瘤細(xì)胞 |
生長(zhǎng)培養(yǎng)基 | RPMI-1640+10% FBS+1% P/S |
推薦傳代比例 | 5×10^5-1×10^6cells/mL |
推薦換液頻率 | 2~3次/周 |
倍增時(shí)間 | ~40小時(shí) |
凍存條件 | 凍存液:55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO溫度:液氮 |
培養(yǎng)條件 | 氣相:空氣,95%;CO2,5%溫度:37℃ |
基因表達(dá)情況 | IgG+; Kappa+; IgM-; IgA-; IgD-; Lambda-; This cell line has relatively high expression levels of Bax; Bak; AIF; high caspase-9 activity. |
注意事項(xiàng) | 該細(xì)胞為懸浮細(xì)胞,請(qǐng)注意離心收集細(xì)胞懸液;請(qǐng)勿直接倒掉細(xì)胞培養(yǎng)液。 |
保藏機(jī)構(gòu) | ATCC; CRL-2957 DSMZ; ACC-495 |
STR鑒定
STR位點(diǎn)信息
Amelogenin X,Y CSF1PO 12 D2S1338 17,19 D3S1358 15,16 D5S818 11,12 D7S820 8,11 D8S1179 13 D13S317 12 D16S539 11,13 D18S51 14,16 D19S433 14,15.2 D21S11 29,31 FGA 19,21 PentaD 9,14 PentaE 7,12 TH01 6,9.3 TPOX 9,11 vWA 18,19 D6S1043 11 D12S391 18,20 D2S441 11,14
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